| Project ID | Title | Publication | Instrument | Description | Notes |
|---|---|---|---|---|---|
| PRJNA341486 | small RNA and RNA profiling in Triticum turgidum and Triticum aestivum | - | Illumina HiSeq 2500 | Identification of key genes for wax production. | Key gene |
| Project ID | Title | Publication | Instrument | Description | Notes |
|---|---|---|---|---|---|
| PRJNA929049 | miRNA sequencing of three Turkish wheat species | Diverse expression pattern of wheat transcription factors against abiotic stresses in wheat species | Illumina HiSeq 2000 | This is a comparison study about miRNA libraries of three Turkish wheat cultivars in different ploidy levels (Triticum aestivum cv. Yuregir-89, Triticum turgidum cv. Kiziltan-91 and Triticum monococcum). In this project, it was aimed to reveal the miRNAs that play a role in drought stress tolerance by investigating the differences in miRNA expression profiles under drought stress among one drought tolerant (Kiziltan-91), another moderately drought tolerant (Yuregir-89) and ancestral wheat species. For this purpose, total RNA isolation was performed by collecting root and leaf samples from control and stressed plants and sRNA-Seq was performed using next generation sequencing (NGS) technology. | Drought stress tolerance |
| PRJNA285254 | Genome-wide identification of differentially expressed microRNAs in leaves and the developing head of four durum genotypes during water deficit stress | Genome-Wide Identification of MicroRNAs in Leaves and the Developing Head of Four Durum Genotypes during Water Deficit Stress | Illumina HiSeq 2500 | MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. Yield loss in durum wheat can be exacerbated due to minimal rainfall in the early reproductive stages of development during Spring in Australia. This study describes genotypic differences in the miRNAome between water deficit tolerant/sensitive durum, using flag leaf and developing head tissue, and more specifically identifies miRNAs associated with water deficit stress. Results: Small RNA libraries (96 in total) were constructed from flag leaf and developing head tissues of four durum genotypes (Tamaroi, Yawa, EGA Bellaroi, Tjilkuri), with or without water deficit stress. Illumina sequencing and subsequent analysis detected 110 conserved miRNAs and 159 novel candidate miRNA hairpins. Statistical analysis of the abundance of sequencing reads revealed 66 conserved miRNAs and five novel miRNA hairpins showing differential expression under water deficit stress. During stress, several conserved and novel miRNAs showed unambiguous inverted regulatory profiles between the durum genotypes studied. Several miRNAs were also identified to have different abundance in the flag leaf compared to the developing head regardless of treatment. Predicted mRNA targets from four novel durum miRNAs were characterised using Gene Ontology (GO) which revealed functions common to stress responses and plant development. Conclusion: For the first time, we present a comprehensive study of the miRNA transcriptome of flag leaf and developing head tissues in different durum genotypes under water deficit stress. The identification of differentially expressed miRNAs provides molecular evidence that miRNAs are potential determinants of water stress tolerance in durum wheat. GO analysis of predicted targets contributes to the understanding of genotype-specific physiological responses leading to stress tolerance capacity. Further functional analysis of specific stress responsive miRNAs identified, and their interaction with mRNA targets is ongoing and will assist in developing future durum wheat varieties with enhanced water deficit stress tolerance. Overall design: A total of 96 small RNA libraries were analysed [4 durum genotypes * 2 tissue types * 2 treatment groups (control and water stress) * 6 biological replicates] in this study. Please see Methods, Liu et al.(2015) | Water deficit stress |
| PRJNA310686 | Triticum turgidum subsp. durum transcriptome sequences | Durum wheat miRNAs in response to nitrogen starvation at the grain filling stage | Illumina Genome Analyzer II | Discovery and analysis of microRNAs in durum wheat. | Nitrogen starvation |
| PRJNA600522 | Transgenerational effects of water-deficit and heat stress on germination and seedling vigour - new insights from durum wheat microRNAs (durum wheat) | Transgenerational Effects of Water-Deficit and Heat Stress on Germination and Seedling Vigour-New Insights from Durum Wheat microRNAs | Illumina HiSeq 2500 | Water deficiency and heat stress can severely limit crop production and quality. Stress imposed on the parents during reproduction could have transgenerational effects on their progeny. Seeds with different origins can vary significantly in germination time-course and early growth. Here, we investigated how water-deficit and heat stress on parental durum wheat plants affected seedling establishment of the subsequent generation. One stress-tolerant and one stress-sensitive Australian durum genotype were used. Seeds were collected from parents with or without exposure to stress during reproduction. Generally stress on the previous generation negatively affected seed germination and seedling vigour, but to a lesser extent in the tolerant variety. Small RNA sequencing utilising the new durum genome assembly has revealed significant differences in microRNA (miRNA) expression in the two genotypes. A bioinformatics approach was used to identify multiple miRNA targets which have critical molecular functions in stress adaptation and plant development and could therefore contribute to the phenotypic differences observed. Our data provides the first confirmation of the transgenerational effects of reproductive-stage stress on germination and seedling establishment in durum wheat. New insights gained on the epigenetic level indicate that durum miRNAs could be key factors in optimising seed vigour for superior breeding germplasm and/or varieties. Overall design: Examine the seedling miRNA population from two seed source of two genotypes. | Water deficiency and heat stress |
| PRJNA641098 | Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat (durum wheat) | Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat | Illumina NovaSeq 6000 | Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. Analysis of 120 sRNA libraries identified 523 microRNAs (miRNAs), of which 55 were novel. Differentially expressed miRNAs (DEMs) were identified that had significantly altered expression subject to stress type, genotype, and time-point. Transcriptome sequencing identified 49,436 genes, with differentially expressed genes (DEGs) linked to processes associated with hormone homeostasis, photosynthesis, and signaling. With the first durum wheat degradome report, over 100,000 transcript target sites were characterized, and new miRNA-mRNA regulatory pairs were discovered. Integrated omics analysis identified key miRNA-mRNA modules (particularly, novel pairs of miRNAs and transcription factors) with antagonistic regulatory patterns subject to different stresses. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis revealed significant roles in plant growth and stress adaptation. Our research provides novel and fundamental knowledge, at the whole-genome level, for transcriptional and post-transcriptional stress regulation in durum wheat. Overall design: Analysis of small RNA transcriptome in 120 samples (2 genotypes x 4 treatments x 5 time-points x 3 biological replicates); Analysis of mRNA transcriptome in eight libraries (2 genotypes x 4 treatments x 1 time-point x 1 pool of 3 biological replicates); Analysis of mRNA degradome in eight libraries (2 genotypes x 4 treatments x 1 time-point x 1 pool of 3 biological replicates). | Water-deficiency and heat stress |
| PRJNA644573 | Multi-omics analysis of small RNA, transcriptome and degradome in T. turgidum - regulatory networks of grain development and abiotic stress response (durum wheat) | Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome in T. turgidum-Regulatory Networks of Grain Development and Abiotic Stress Response | Illumina NovaSeq 6000 | Crop reproduction is highly sensitive to water-deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (T. turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome and degradome in T. turgidum developing grains, under single and combined water-deficit and heat stress. We identified 690 microRNAs (miRNAs), with 84 being novel, from 118 sRNA libraries. Complete profiles of differentially expressed miRNA (DEMs) specific to genotypes, stress types and different reproductive time-points are provided. The first degradome-seq report for developing durum grains discovered a significant number of new target genes regulated by miRNAs post-transcriptionally. Transcriptome-seq profiled 53,146 T. turgidum genes, with differentially expressed genes (DEGs) enriched in functional categories such as nutrient metabolism, cellular differentiation, transport, reproductive development and hormone transduction pathways. miRNA-mRNA networks that affect grain characteristics such as starch synthesis and protein metabolism were constructed, based on integrated analysis of the three omics. This study provides a substantial amount of novel information on the post-transcriptional networks in T. turgidum grains, which will facilitate innovations for breeding programs aiming to improve crop resilience and grain quality. Overall design: Analysis of small RNA transcriptome in 118 samples (2 genotypes x4 treatments x5 time-points x3 biological replicates); Analysis of mRNA transcriptome in eight libraries (2 genotypes x4 treatments x1 time-point x1 pool of 3 biological replicates); Analysis of mRNA degradome in eight libraries (2 genotypes x4 treatments x1 time-point x1 pool of 3 biological replicates). | Water-deficiency and heat stress |
| PRJNA680308 | Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat | Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat | Illumina NovaSeq 6000 | Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on the parental plants during reproduction could have transgenerational effects on the progenies. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in the progenies. Parental water-deficit stress had positive impacts on the progenies in certain traits like harvest index and protein content in given genotype. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter- generational stress. Integrated analysis based on the three omics revealed the significant biological interactions between stress-responsive miRNA and targets, with possible contributions towards transgenerational stress tolerance via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained on the molecular level indicate that key miRNA-mRNA modules are potential. | Water-deficiency stress |
| PRJNA706013 | Nitrogen starvation-responsive microRNAs are affected by transgenerational stress in durum wheat seedlings (durum wheat) | Nitrogen Starvation-Responsive MicroRNAs Are Affected by Transgenerational Stress in Durum Wheat Seedlings | Illumina NovaSeq 6000 | Stress events have transgenerational effects that influence plant growth in the subsequent generation. In Mediterranean regions, water-deficit and heat (WH) stress is a frequent issue that negatively affects crop yield and quality. Nitrogen (N) is an essential plant macronutrient and often a yield-limiting factor for crops. Here, the response of durum wheat seedlings to N starvation under the transgenerational effects of WH stress were investigated in two genotypes. Both genotypes showed significant reduction in seedling height, leaf number, shoot and root weight (fresh and dry), primary root length and chlorophyll content under N starvation stress. However, in the WH stress-tolerant genotype, the reduction rate of most traits were lower in progeny from the stressed parents than progeny from the control parents. Small RNA sequencing identified 1,534 microRNAs in different treatment groups. Differentially expressed microRNAs (DEMs) were characterized subject to N starvation, parental stress and genotype factors, with their target genes identified in silico. GO and KEGG enrichment analyses revealed the biological functions associated with DEM-target modules in stress adaptation processes, which could contribute to the phenotypic differences observed in two genotypes. The study provides the first evidence of the transgenerational effects of WH stress on N starvation response in durum wheat. Overall design: Analysis of small RNA transcriptome in eight biological pools (two genotypes by two parental treatment by two progeny treatment). | Nitrogen starvation |
| PRJNA719092 | Small RNA, transcriptome and degradome analysis of the transgenerational heat stress response network in durum wheat (durum wheat) | Small RNA, Transcriptome and Degradome Analysis of the Transgenerational Heat Stress Response Network in Durum Wheat | Illumina NovaSeq 6000 | Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. Parental heat stress had positive influence on the offspring under re-occurring stress for traits like chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome, and mRNA degradome provided the first description of the molecular networks mediating heat stress adaption under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes identified subject to the progeny treatment, parental treatment and tissue type factors. Gene Ontology and KEGG pathway annotation of stress responsive microRNAs-mRNA modules provided further information on their functional roles in biological processes like hormone homeostasis, signal transduction, and protein stabilization. Our results provide new sights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermos-tolerance in breeding. Overall design: Analysis of small RNA transcriptome, mRNA transcriptome and mRNA degradome in eight biological pools (four treatment groups by two tissue types). | Heat stress |
| Project ID | Title | Publication | Instrument | Description | Notes |
|---|---|---|---|---|---|
| PRJNA473404 | Svevo Durum Wheat | Durum wheat genome highlights past domestication signatures and future improvement targets | Illumina HiSeq 2000 | A reference genome sequence for Svevo durum wheat has been produced and annotated. | TdHMA3-B1 |
| Run | Library Selection | Library Name | genotype | Stage | Tissue | Phenotype | Sample Name | Geographic Location Name | Replicate |
|---|---|---|---|---|---|---|---|---|---|
| SRR4114480 | RANDOM | 7294_sRNASeq | 7294 | booting | Leaf sheath | glaucous | 7294_sRNASeq | Canada: Saskatchewan | - |
| SRR4114479 | RANDOM | 7293_sRNASeq | 7293 | booting | Leaf sheath | glaucous | 7293_sRNASeq | Canada: Saskatchewan | - |
| SRR4114478 | RANDOM | 7290_sRNASeq | 7290 | booting | Leaf sheath | glaucous | 7290_sRNASeq | Canada: Saskatchewan | - |
| SRR4114477 | RANDOM | 7289_sRNASeq | 7289 | booting | Leaf sheath | glaucous | 7289_sRNASeq | Canada: Saskatchewan | - |
| SRR4114476 | RANDOM | 7287_sRNASeq | 7287 | booting | Leaf sheath | non-glaucous | 7287_sRNASeq | Canada: Saskatchewan | - |
| SRR4114475 | RANDOM | 7285_sRNASeq | 7285 | booting | Leaf sheath | non-glaucous | 7285_sRNASeq | Canada: Saskatchewan | - |
| SRR4114473 | RANDOM | 7282_sRNASeq | 7282 | booting | Leaf sheath | non-glaucous | 7282_sRNASeq | Canada: Saskatchewan | - |
| SRR4114472 | RANDOM | 7281_sRNASeq | 7281 | booting | Leaf sheath | non-glaucous | 7281_sRNASeq | Canada: Saskatchewan | - |
| SRR4114471 | RANDOM | 7279_sRNASeq | 7279 | booting | Leaf sheath | non-glaucous | 7279_sRNASeq | Canada: Saskatchewan | - |
| Run | Library Selection | Library Name | Ecotype | Stage | Tissue | Cultivar | Sample Name | Geographic Location Name |
|---|---|---|---|---|---|---|---|---|
| SRR23262621 | cDNA | TtSRoot | Turkey | Tillering | Root | Kiziltan-91 | TtSRoot | Turkey: Kastamonu |
| SRR23262622 | cDNA | TtCRoot | Turkey | Tillering | Root | Kiziltan-91 | TtCRoot | Turkey: Kastamonu |
| SRR23262623 | cDNA | TtSLeaf | Turkey | Tillering | Leaf | Kiziltan-91 | TtSLeaf | Turkey: Kastamonu |
| SRR23262624 | cDNA | TtCLeaf | Turkey | Tillering | Leaf | Kiziltan-91 | TtCLeaf | Turkey: Kastamonu |
| Experiment Accession | Run | Library Selection | Tissue | sub_species | variety_name | Sample Name | Treatment |
|---|---|---|---|---|---|---|---|
| SRX1042025 | SRR2043911 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698216 | Water deficit stress treatment group (WG) |
| SRX1042024 | SRR2043909 SRR2043910 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698215 | Water deficit stress treatment group (WG) |
| SRX1042023 | SRR2043907 SRR2043908 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698214 | Water deficit stress treatment group (WG) |
| SRX1042022 | SRR2043905 SRR2043906 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698213 | Water deficit stress treatment group (WG) |
| SRX1042021 | SRR2043903 SRR2043904 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698212 | Water deficit stress treatment group (WG) |
| SRX1042020 | SRR2043901 SRR2043902 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698211 | Water deficit stress treatment group (WG) |
| SRX1042019 | SRR2043899 SRR2043900 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698210 | Control Group (CG) |
| SRX1042018 | SRR2043897 SRR2043898 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698209 | Control Group (CG) |
| SRX1042017 | SRR2043895 SRR2043896 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698208 | Control Group (CG) |
| SRX1042016 | SRR2043893 SRR2043894 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698207 | Control Group (CG) |
| SRX1042015 | SRR2043892 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698206 | Control Group (CG) |
| SRX1042014 | SRR2043890 SRR2043891 | size fractionation | Developing head (H) | durum | Tjilkuri | GSM1698205 | Control Group (CG) |
| SRX1042013 | SRR2043888 SRR2043889 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698204 | Water deficit stress treatment group (WG) |
| SRX1042012 | SRR2043887 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698203 | Water deficit stress treatment group (WG) |
| SRX1042011 | SRR2043885 SRR2043886 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698202 | Water deficit stress treatment group (WG) |
| SRX1042010 | SRR2043883 SRR2043884 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698201 | Water deficit stress treatment group (WG) |
| SRX1042009 | SRR2043881 SRR2043882 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698200 | Water deficit stress treatment group (WG) |
| SRX1042008 | SRR2043879 SRR2043880 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698199 | Water deficit stress treatment group (WG) |
| SRX1042007 | SRR2043877 SRR2043878 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698198 | Control Group (CG) |
| SRX1042006 | SRR2043875 SRR2043876 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698197 | Control Group (CG) |
| SRX1042005 | SRR2043873 SRR2043874 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698196 | Control Group (CG) |
| SRX1042004 | SRR2043872 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698195 | Control Group (CG) |
| SRX1042003 | SRR2043871 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698194 | Control Group (CG) |
| SRX1042002 | SRR2043869 SRR2043870 | size fractionation | Flag leaf (FL) | durum | Tjilkuri | GSM1698193 | Control Group (CG) |
| SRX1042001 | SRR2043867 SRR2043868 | size fractionation | Developing head (H) | durum | Yawa | GSM1698192 | Water deficit stress treatment group (WG) |
| SRX1042000 | SRR2043865 SRR2043866 | size fractionation | Developing head (H) | durum | Yawa | GSM1698191 | Water deficit stress treatment group (WG) |
| SRX1041999 | SRR2043863 SRR2043864 | size fractionation | Developing head (H) | durum | Yawa | GSM1698190 | Water deficit stress treatment group (WG) |
| SRX1041998 | SRR2043862 | size fractionation | Developing head (H) | durum | Yawa | GSM1698189 | Water deficit stress treatment group (WG) |
| SRX1041997 | SRR2043860 SRR2043861 | size fractionation | Developing head (H) | durum | Yawa | GSM1698188 | Water deficit stress treatment group (WG) |
| SRX1041996 | SRR2043858 SRR2043859 | size fractionation | Developing head (H) | durum | Yawa | GSM1698187 | Water deficit stress treatment group (WG) |
| SRX1041995 | SRR2043857 | size fractionation | Developing head (H) | durum | Yawa | GSM1698186 | Control Group (CG) |
| SRX1041994 | SRR2043855 SRR2043856 | size fractionation | Developing head (H) | durum | Yawa | GSM1698185 | Control Group (CG) |
| SRX1041993 | SRR2043853 SRR2043854 | size fractionation | Developing head (H) | durum | Yawa | GSM1698184 | Control Group (CG) |
| SRX1041992 | SRR2043851 SRR2043852 | size fractionation | Developing head (H) | durum | Yawa | GSM1698183 | Control Group (CG) |
| SRX1041991 | SRR2043849 SRR2043850 | size fractionation | Developing head (H) | durum | Yawa | GSM1698182 | Control Group (CG) |
| SRX1041990 | SRR2043847 SRR2043848 | size fractionation | Developing head (H) | durum | Yawa | GSM1698181 | Control Group (CG) |
| SRX1041989 | SRR2043845 SRR2043846 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698180 | Water deficit stress treatment group (WG) |
| SRX1041988 | SRR2043843 SRR2043844 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698179 | Water deficit stress treatment group (WG) |
| SRX1041987 | SRR2043841 SRR2043842 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698178 | Water deficit stress treatment group (WG) |
| SRX1041986 | SRR2043839 SRR2043840 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698177 | Water deficit stress treatment group (WG) |
| SRX1041985 | SRR2043837 SRR2043838 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698176 | Water deficit stress treatment group (WG) |
| SRX1041984 | SRR2043835 SRR2043836 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698175 | Water deficit stress treatment group (WG) |
| SRX1041983 | SRR2043833 SRR2043834 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698174 | Control Group (CG) |
| SRX1041982 | SRR2043831 SRR2043832 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698173 | Control Group (CG) |
| SRX1041981 | SRR2043830 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698172 | Control Group (CG) |
| SRX1041980 | SRR2043828 SRR2043829 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698171 | Control Group (CG) |
| SRX1041979 | SRR2043826 SRR2043827 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698170 | Control Group (CG) |
| SRX1041978 | SRR2043825 | size fractionation | Flag leaf (FL) | durum | Yawa | GSM1698169 | Control Group (CG) |
| SRX1041977 | SRR2043823 SRR2043824 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698168 | Water deficit stress treatment group (WG) |
| SRX1041976 | SRR2043821 SRR2043822 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698167 | Water deficit stress treatment group (WG) |
| SRX1041975 | SRR2043819 SRR2043820 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698166 | Water deficit stress treatment group (WG) |
| SRX1041974 | SRR2043818 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698165 | Water deficit stress treatment group (WG) |
| SRX1041973 | SRR2043816 SRR2043817 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698164 | Water deficit stress treatment group (WG) |
| SRX1041972 | SRR2043814 SRR2043815 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698163 | Water deficit stress treatment group (WG) |
| SRX1041971 | SRR2043813 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698162 | Control Group (CG) |
| SRX1041970 | SRR2043811 SRR2043812 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698161 | Control Group (CG) |
| SRX1041969 | SRR2043809 SRR2043810 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698160 | Control Group (CG) |
| SRX1041968 | SRR2043807 SRR2043808 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698159 | Control Group (CG) |
| SRX1041967 | SRR2043805 SRR2043806 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698158 | Control Group (CG) |
| SRX1041966 | SRR2043803 SRR2043804 | size fractionation | Developing head (H) | durum | EGA Bellaroi | GSM1698157 | Control Group (CG) |
| SRX1041965 | SRR2043801 SRR2043802 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698156 | Water deficit stress treatment group (WG) |
| SRX1041964 | SRR2043800 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698155 | Water deficit stress treatment group (WG) |
| SRX1041963 | SRR2043799 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698154 | Water deficit stress treatment group (WG) |
| SRX1041962 | SRR2043797 SRR2043798 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698153 | Water deficit stress treatment group (WG) |
| SRX1041961 | SRR2043796 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698152 | Water deficit stress treatment group (WG) |
| SRX1041960 | SRR2043794 SRR2043795 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698151 | Water deficit stress treatment group (WG) |
| SRX1041959 | SRR2043793 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698150 | Control Group (CG) |
| SRX1041958 | SRR2043791 SRR2043792 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698149 | Control Group (CG) |
| SRX1041957 | SRR2043789 SRR2043790 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698148 | Control Group (CG) |
| SRX1041956 | SRR2043788 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698147 | Control Group (CG) |
| SRX1041955 | SRR2043787 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698146 | Control Group (CG) |
| SRX1041954 | SRR2043786 | size fractionation | Flag leaf (FL) | durum | EGA Bellaroi | GSM1698145 | Control Group (CG) |
| SRX1041953 | SRR2043784 SRR2043785 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698144 | Water deficit stress treatment group (WG) |
| SRX1041952 | SRR2043782 SRR2043783 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698143 | Water deficit stress treatment group (WG) |
| SRX1041951 | SRR2043780 SRR2043781 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698142 | Water deficit stress treatment group (WG) |
| SRX1041950 | SRR2043779 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698141 | Water deficit stress treatment group (WG) |
| SRX1041949 | SRR2043778 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698140 | Water deficit stress treatment group (WG) |
| SRX1041948 | SRR2043776 SRR2043777 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698139 | Water deficit stress treatment group (WG) |
| SRX1041947 | SRR2043775 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698138 | Control Group (CG) |
| SRX1041946 | SRR2043773 SRR2043774 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698137 | Control Group (CG) |
| SRX1041945 | SRR2043771 SRR2043772 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698136 | Control Group (CG) |
| SRX1041944 | SRR2043769 SRR2043770 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698135 | Control Group (CG) |
| SRX1041943 | SRR2043767 SRR2043768 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698134 | Control Group (CG) |
| SRX1041942 | SRR2043766 | size fractionation | Developing head (H) | durum | Tamaroi | GSM1698133 | Control Group (CG) |
| SRX1041941 | SRR2043765 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698132 | Water deficit stress treatment group (WG) |
| SRX1041940 | SRR2043763 SRR2043764 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698131 | Water deficit stress treatment group (WG) |
| SRX1041939 | SRR2043761 SRR2043762 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698130 | Water deficit stress treatment group (WG) |
| SRX1041938 | SRR2043759 SRR2043760 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698129 | Water deficit stress treatment group (WG) |
| SRX1041937 | SRR2043757 SRR2043758 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698128 | Water deficit stress treatment group (WG) |
| SRX1041936 | SRR2043755 SRR2043756 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698127 | Water deficit stress treatment group (WG) |
| SRX1041935 | SRR2043754 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698126 | Control Group (CG) |
| SRX1041934 | SRR2043752 SRR2043753 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698125 | Control Group (CG) |
| SRX1041933 | SRR2043750 SRR2043751 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698124 | Control Group (CG) |
| SRX1041932 | SRR2043748 SRR2043749 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698123 | Control Group (CG) |
| SRX1041931 | SRR2043746 SRR2043747 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698122 | Control Group (CG) |
| SRX1041930 | SRR2043745 | size fractionation | Flag leaf (FL) | durum | Tamaroi | GSM1698121 | Control Group (CG) |
| Run | Library Name | Library Selection | Stage | Tissue | sub_species | Cultivar | Sample Name | Geographic Location Name | Treatment |
|---|---|---|---|---|---|---|---|---|---|
| SRR6649861 | LIB_02 | size fractionation | seediling | leaves | durum | Ciccio | LIB02_Ciccio_F0_sRNA | Italy | Nitrogen deprivation |
| SRR6649862 | LIB_01 | size fractionation | seediling | roots | durum | Ciccio | LIB01_Ciccio_R0_sRNA | Italy | Nitrogen deprivation |
| SRR6649863 | LIB_04 | size fractionation | seediling | leaves | durum | Ciccio | LIB04_Ciccio_F2_sRNA | Italy | - |
| SRR6649864 | LIB_03 | size fractionation | seediling | roots | durum | Ciccio | LIB03_Ciccio_R2_sRNA | Italy | - |
| SRR6649865 | LIB_06 | size fractionation | seediling | leaves | durum | Svevo | LIB06_Svevo_F0_sRNA | Italy | Nitrogen deprivation |
| SRR6649866 | LIB_05 | size fractionation | seediling | roots | durum | Svevo | LIB05_Svevo_R0_sRNA | Italy | Nitrogen deprivation |
| SRR6649867 | LIB_08 | size fractionation | seediling | leaves | durum | Svevo | LIB08_Svevo_F2_sRNA | Italy | - |
| SRR6649868 | LIB_07 | size fractionation | seediling | roots | durum | Svevo | LIB07_Svevo_R2_sRNA | Italy | - |
| SRR3607662 | LIB_11 | size fractionation | late milk | root,leaf,flag leaf,spike | durum | Svevo | LIBRARY 11 smallRNA from Triticum turgidum subsp. durum - Svevo (0 mM N) | Italy | Nitrogen deprivation |
| SRR3603231 | LIB_09 | size fractionation | late milk | root,leaf,flag leaf,spike | durum | Ciccio | LIBRARY 09 smallRNA from Triticum turgidum subsp. durum - Ciccio (0 mM N) | Italy | Nitrogen deprivation |
| SRR3147015 | PRJNA310686 | size fractionation | Late milk - Z77 | small RNA pool from roots,leaves,flag leaves,spikes | durum | Svevo | Small RNA durum wheat LIBRARY 12 | Italy | - |
| SRR3144594 | Library 10 durum wheat Ciccio | RANDOM | Late milk - Z77 | small RNA pool from roots,leaves,flag leaves,spikes | durum | Ciccio | Small RNA durum wheat LIBRARY 10 | Italy | - |
| Run | Library Selection | Genotype | Tissue | sub_species | Sample Name | Treatment |
|---|---|---|---|---|---|---|
| SRR10870722 | size fractionation | Line 6 (UAD1301020-8) | shoot | durum | GSM4259761 | Water-deficit and heat stress |
| SRR10870721 | size fractionation | Line 6 (UAD1301020-8) | shoot | durum | GSM4259760 | Control |
| SRR10870720 | size fractionation | DBA Aurora | shoot | durum | GSM4259759 | Water-deficit and heat stress |
| SRR10870719 | size fractionation | DBA Aurora | shoot | durum | GSM4259758 | Control |
| Run | Library Selection | Genotype | sub_species | Sample Name | Treatment | Replicate |
|---|---|---|---|---|---|---|
| SRR12067466 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630931 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067465 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630930 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067464 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630929 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067463 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630928 | Heat stress (HS) | Rep3 |
| SRR12067462 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630927 | Heat stress (HS) | Rep2 |
| SRR12067461 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630926 | Heat stress (HS) | Rep1 |
| SRR12067460 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630925 | Water-deficit stress (WS) | Rep3 |
| SRR12067459 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630924 | Water-deficit stress (WS) | Rep2 |
| SRR12067458 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630923 | Water-deficit stress (WS) | Rep1 |
| SRR12067457 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630922 | Control group (CG) | Rep3 |
| SRR12067456 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630921 | Control group (CG) | Rep2 |
| SRR12067455 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630920 | Control group (CG) | Rep1 |
| SRR12067454 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630919 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067453 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630918 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067452 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630917 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067451 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630916 | Heat stress (HS) | Rep3 |
| SRR12067450 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630915 | Heat stress (HS) | Rep2 |
| SRR12067449 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630914 | Heat stress (HS) | Rep1 |
| SRR12067448 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630913 | Water-deficit stress (WS) | Rep3 |
| SRR12067447 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630912 | Water-deficit stress (WS) | Rep2 |
| SRR12067446 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630911 | Water-deficit stress (WS) | Rep1 |
| SRR12067445 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630910 | Control group (CG) | Rep3 |
| SRR12067444 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630909 | Control group (CG) | Rep2 |
| SRR12067443 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630908 | Control group (CG) | Rep1 |
| SRR12067442 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630907 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067441 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630906 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067440 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630905 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067439 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630904 | Heat stress (HS) | Rep3 |
| SRR12067438 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630903 | Heat stress (HS) | Rep2 |
| SRR12067437 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630902 | Heat stress (HS) | Rep1 |
| SRR12067436 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630901 | Water-deficit stress (WS) | Rep3 |
| SRR12067435 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630900 | Water-deficit stress (WS) | Rep2 |
| SRR12067434 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630899 | Water-deficit stress (WS) | Rep1 |
| SRR12067433 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630898 | Control group (CG) | Rep3 |
| SRR12067432 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630897 | Control group (CG) | Rep2 |
| SRR12067431 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630896 | Control group (CG) | Rep1 |
| SRR12067430 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630895 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067429 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630894 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067428 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630893 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067427 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630892 | Heat stress (HS) | Rep3 |
| SRR12067426 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630891 | Heat stress (HS) | Rep2 |
| SRR12067425 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630890 | Heat stress (HS) | Rep1 |
| SRR12067424 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630889 | Water-deficit stress (WS) | Rep3 |
| SRR12067423 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630888 | Water-deficit stress (WS) | Rep2 |
| SRR12067422 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630887 | Water-deficit stress (WS) | Rep1 |
| SRR12067421 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630886 | Control group (CG) | Rep3 |
| SRR12067420 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630885 | Control group (CG) | Rep2 |
| SRR12067419 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630884 | Control group (CG) | Rep1 |
| SRR12067418 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630883 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067417 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630882 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067416 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630881 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067415 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630880 | Heat stress (HS) | Rep3 |
| SRR12067414 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630879 | Heat stress (HS) | Rep2 |
| SRR12067413 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630878 | Heat stress (HS) | Rep1 |
| SRR12067412 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630877 | Water-deficit stress (WS) | Rep3 |
| SRR12067411 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630876 | Water-deficit stress (WS) | Rep2 |
| SRR12067410 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630875 | Water-deficit stress (WS) | Rep1 |
| SRR12067409 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630874 | Control group (CG) | Rep3 |
| SRR12067408 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630873 | Control group (CG) | Rep2 |
| SRR12067407 | size fractionation | L6 (Stres-sensitive) | durum | GSM4630872 | Control group (CG) | Rep1 |
| SRR12067406 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630871 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067405 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630870 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067404 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630869 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067403 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630868 | Heat stress (HS) | Rep3 |
| SRR12067402 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630867 | Heat stress (HS) | Rep2 |
| SRR12067401 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630866 | Heat stress (HS) | Rep1 |
| SRR12067400 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630865 | Water-deficit stress (WS) | Rep3 |
| SRR12067399 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630864 | Water-deficit stress (WS) | Rep2 |
| SRR12067398 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630863 | Water-deficit stress (WS) | Rep1 |
| SRR12067397 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630862 | Control group (CG) | Rep3 |
| SRR12067396 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630861 | Control group (CG) | Rep2 |
| SRR12067395 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630860 | Control group (CG) | Rep1 |
| SRR12067394 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630859 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067393 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630858 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067392 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630857 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067391 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630856 | Heat stress (HS) | Rep3 |
| SRR12067390 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630855 | Heat stress (HS) | Rep2 |
| SRR12067389 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630854 | Heat stress (HS) | Rep1 |
| SRR12067388 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630853 | Water-deficit stress (WS) | Rep3 |
| SRR12067387 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630852 | Water-deficit stress (WS) | Rep2 |
| SRR12067386 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630851 | Water-deficit stress (WS) | Rep1 |
| SRR12067385 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630850 | Control group (CG) | Rep3 |
| SRR12067384 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630849 | Control group (CG) | Rep2 |
| SRR12067383 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630848 | Control group (CG) | Rep1 |
| SRR12067382 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630847 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067381 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630846 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067380 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630845 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067379 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630844 | Heat stress (HS) | Rep3 |
| SRR12067378 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630843 | Heat stress (HS) | Rep2 |
| SRR12067377 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630842 | Heat stress (HS) | Rep1 |
| SRR12067376 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630841 | Water-deficit stress (WS) | Rep3 |
| SRR12067375 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630840 | Water-deficit stress (WS) | Rep2 |
| SRR12067374 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630839 | Water-deficit stress (WS) | Rep1 |
| SRR12067373 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630838 | Control group (CG) | Rep3 |
| SRR12067372 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630837 | Control group (CG) | Rep2 |
| SRR12067371 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630836 | Control group (CG) | Rep1 |
| SRR12067370 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630835 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067369 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630834 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067368 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630833 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067367 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630832 | Heat stress (HS) | Rep3 |
| SRR12067366 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630831 | Heat stress (HS) | Rep2 |
| SRR12067365 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630830 | Heat stress (HS) | Rep1 |
| SRR12067364 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630829 | Water-deficit stress (WS) | Rep3 |
| SRR12067363 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630828 | Water-deficit stress (WS) | Rep2 |
| SRR12067362 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630827 | Water-deficit stress (WS) | Rep1 |
| SRR12067361 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630826 | Control group (CG) | Rep3 |
| SRR12067360 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630825 | Control group (CG) | Rep2 |
| SRR12067359 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630824 | Control group (CG) | Rep1 |
| SRR12067358 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630823 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12067357 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630822 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12067356 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630821 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12067355 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630820 | Heat stress (HS) | Rep3 |
| SRR12067354 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630819 | Heat stress (HS) | Rep2 |
| SRR12067353 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630818 | Heat stress (HS) | Rep1 |
| SRR12067352 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630817 | Water-deficit stress (WS) | Rep3 |
| SRR12067351 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630816 | Water-deficit stress (WS) | Rep2 |
| SRR12067350 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630815 | Water-deficit stress (WS) | Rep1 |
| SRR12067349 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630814 | Control group (CG) | Rep3 |
| SRR12067348 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630813 | Control group (CG) | Rep2 |
| SRR12067347 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4630812 | Control group (CG) | Rep1 |
| Run | Library Selection | Genotype | sub_species | Sample Name | Treatment | Replicate |
|---|---|---|---|---|---|---|
| SRR12164786 | size fractionation | - | durum | GSM4658665 | - | Rep1 |
| SRR12164785 | size fractionation | - | durum | GSM4658664 | - | Rep2 |
| SRR12164784 | size fractionation | - | durum | GSM4658663 | - | Rep1 |
| SRR12164783 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658662 | Control group (CG) | Rep3 |
| SRR12164782 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658661 | Control group (CG) | Rep2 |
| SRR12164781 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658660 | Control group (CG) | Rep1 |
| SRR12164780 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658659 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164779 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658658 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164778 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658657 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164777 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658656 | Heat stress (HS) | Rep3 |
| SRR12164776 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658655 | Heat stress (HS) | Rep2 |
| SRR12164775 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658654 | Heat stress (HS) | Rep1 |
| SRR12164774 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658653 | Water-deficit stress (WS) | Rep3 |
| SRR12164773 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658652 | Water-deficit stress (WS) | Rep2 |
| SRR12164772 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658651 | Water-deficit stress (WS) | Rep1 |
| SRR12164771 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658650 | Control group (CG) | Rep3 |
| SRR12164770 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658649 | Control group (CG) | Rep2 |
| SRR12164769 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658648 | Control group (CG) | Rep1 |
| SRR12164768 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658647 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164767 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658646 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164766 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658645 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164765 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658644 | Heat stress (HS) | Rep3 |
| SRR12164764 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658643 | Heat stress (HS) | Rep2 |
| SRR12164763 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658642 | Heat stress (HS) | Rep1 |
| SRR12164762 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658641 | Water-deficit stress (WS) | Rep3 |
| SRR12164761 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658640 | Water-deficit stress (WS) | Rep2 |
| SRR12164760 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658639 | Water-deficit stress (WS) | Rep1 |
| SRR12164759 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658638 | Control group (CG) | Rep3 |
| SRR12164758 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658637 | Control group (CG) | Rep2 |
| SRR12164757 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658636 | Control group (CG) | Rep1 |
| SRR12164756 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658635 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164755 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658634 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164754 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658633 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164753 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658632 | Heat stress (HS) | Rep3 |
| SRR12164752 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658631 | Heat stress (HS) | Rep2 |
| SRR12164751 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658630 | Heat stress (HS) | Rep1 |
| SRR12164750 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658629 | Water-deficit stress (WS) | Rep3 |
| SRR12164749 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658628 | Water-deficit stress (WS) | Rep2 |
| SRR12164748 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658627 | Water-deficit stress (WS) | Rep1 |
| SRR12164747 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658626 | Control group (CG) | Rep3 |
| SRR12164746 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658625 | Control group (CG) | Rep2 |
| SRR12164745 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658624 | Control group (CG) | Rep1 |
| SRR12164744 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658623 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164743 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658622 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164742 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658621 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164741 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658620 | Heat stress (HS) | Rep3 |
| SRR12164740 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658619 | Heat stress (HS) | Rep2 |
| SRR12164739 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658618 | Heat stress (HS) | Rep1 |
| SRR12164738 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658617 | Water-deficit stress (WS) | Rep3 |
| SRR12164737 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658616 | Water-deficit stress (WS) | Rep2 |
| SRR12164736 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658615 | Water-deficit stress (WS) | Rep1 |
| SRR12164735 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658614 | Control group (CG) | Rep3 |
| SRR12164734 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658613 | Control group (CG) | Rep2 |
| SRR12164733 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658612 | Control group (CG) | Rep1 |
| SRR12164732 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658611 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164731 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658610 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164730 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658609 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164729 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658608 | Heat stress (HS) | Rep3 |
| SRR12164728 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658607 | Heat stress (HS) | Rep2 |
| SRR12164727 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658606 | Heat stress (HS) | Rep1 |
| SRR12164726 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658605 | Water-deficit stress (WS) | Rep3 |
| SRR12164725 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658604 | Water-deficit stress (WS) | Rep2 |
| SRR12164724 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658603 | Water-deficit stress (WS) | Rep1 |
| SRR12164723 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658602 | Control group (CG) | Rep3 |
| SRR12164722 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658601 | Control group (CG) | Rep2 |
| SRR12164721 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658600 | Control group (CG) | Rep1 |
| SRR12164720 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658599 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164719 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658598 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164718 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658597 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164717 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658596 | Heat stress (HS) | Rep3 |
| SRR12164716 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658595 | Heat stress (HS) | Rep2 |
| SRR12164715 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658594 | Heat stress (HS) | Rep1 |
| SRR12164714 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658593 | Water-deficit stress (WS) | Rep3 |
| SRR12164713 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658592 | Water-deficit stress (WS) | Rep2 |
| SRR12164712 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658591 | Water-deficit stress (WS) | Rep1 |
| SRR12164711 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658590 | Control group (CG) | Rep3 |
| SRR12164710 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658589 | Control group (CG) | Rep2 |
| SRR12164709 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658588 | Control group (CG) | Rep1 |
| SRR12164708 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658587 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164707 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658586 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164706 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658585 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164705 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658584 | Heat stress (HS) | Rep3 |
| SRR12164704 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658583 | Heat stress (HS) | Rep2 |
| SRR12164703 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658582 | Heat stress (HS) | Rep1 |
| SRR12164702 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658581 | Water-deficit stress (WS) | Rep3 |
| SRR12164701 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658580 | Water-deficit stress (WS) | Rep2 |
| SRR12164700 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658579 | Water-deficit stress (WS) | Rep1 |
| SRR12164699 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658578 | Control group (CG) | Rep3 |
| SRR12164698 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658577 | Control group (CG) | Rep2 |
| SRR12164697 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658576 | Control group (CG) | Rep1 |
| SRR12164696 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658575 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164695 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658574 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164694 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658573 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164693 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658572 | Heat stress (HS) | Rep3 |
| SRR12164692 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658571 | Heat stress (HS) | Rep2 |
| SRR12164691 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658570 | Heat stress (HS) | Rep1 |
| SRR12164690 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658569 | Water-deficit stress (WS) | Rep3 |
| SRR12164689 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658568 | Water-deficit stress (WS) | Rep2 |
| SRR12164688 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658567 | Water-deficit stress (WS) | Rep1 |
| SRR12164687 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658566 | Control group (CG) | Rep3 |
| SRR12164686 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658565 | Control group (CG) | Rep2 |
| SRR12164685 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658564 | Control group (CG) | Rep1 |
| SRR12164684 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658563 | Water-deficit plus heat stress (WH) | Rep3 |
| SRR12164683 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658562 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164682 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658561 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164681 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658560 | Heat stress (HS) | Rep3 |
| SRR12164680 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658559 | Heat stress (HS) | Rep2 |
| SRR12164679 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658558 | Heat stress (HS) | Rep1 |
| SRR12164678 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658557 | Water-deficit stress (WS) | Rep3 |
| SRR12164677 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658556 | Water-deficit stress (WS) | Rep2 |
| SRR12164676 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658555 | Water-deficit stress (WS) | Rep1 |
| SRR12164675 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658554 | Control group (CG) | Rep3 |
| SRR12164790 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658669 | Water-deficit plus heat stress (WH) | Rep2 |
| SRR12164789 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658668 | Water-deficit plus heat stress (WH) | Rep1 |
| SRR12164788 | size fractionation | L6 (Stres-sensitive) | durum | GSM4658667 | Heat stress (HS) | Rep3 |
| SRR12164787 | size fractionation | - | durum | GSM4658666 | - | Rep2 |
| SRR12164674 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658553 | Control group (CG) | Rep2 |
| SRR12164673 | size fractionation | DBA Aurora (Stress-tolerant) | durum | GSM4658552 | Control group (CG) | Rep1 |
| Run | Library Selection | Genotype | sub_species | Sample Name | Treatment |
|---|---|---|---|---|---|
| SRR13114338 | size fractionation | DBA Artemis | durum | GSM4929666 | Water-deficit stress group |
| SRR13114337 | size fractionation | DBA Artemis | durum | GSM4929665 | Control group |
| SRR13114336 | size fractionation | DBA Artemis | durum | GSM4929664 | Water-deficit stress group |
| SRR13114335 | size fractionation | DBA Artemis | durum | GSM4929663 | Control group |
| SRR13114334 | size fractionation | DBA Artemis | durum | GSM4929662 | Water-deficit stress group |
| SRR13114333 | size fractionation | DBA Artemis | durum | GSM4929661 | Control group |
| SRR13114332 | size fractionation | DBA Artemis | durum | GSM4929660 | Water-deficit stress group |
| SRR13114331 | size fractionation | DBA Artemis | durum | GSM4929659 | Control group |
| Run | Library Selection | Genotype | sub_species | Sample Name | Treatment |
|---|---|---|---|---|---|
| SRR13833550 | size fractionation | L6 (UAD1301020-8) | durum | GSM5128902 | Nitrogen starvation group |
| SRR13833549 | size fractionation | L6 (UAD1301020-8) | durum | GSM5128901 | Control group |
| SRR13833548 | size fractionation | L6 (UAD1301020-8) | durum | GSM5128900 | Nitrogen starvation group |
| SRR13833547 | size fractionation | L6 (UAD1301020-8) | durum | GSM5128899 | Control group |
| SRR13833546 | size fractionation | DBA Aurora | durum | GSM5128898 | Nitrogen starvation group |
| SRR13833545 | size fractionation | DBA Aurora | durum | GSM5128897 | Control group |
| SRR13833544 | size fractionation | DBA Aurora | durum | GSM5128896 | Nitrogen starvation group |
| SRR13833543 | size fractionation | DBA Aurora | durum | GSM5128895 | Control group |
| Run | Library Selection | Genotype | Tissue | sub_species | Sample Name | Treatment |
|---|---|---|---|---|---|---|
| SRR14128339 | size fractionation | DBA Aurora | Developing grain tissue | durum | GSM5222605 | Heat stress group |
| SRR14128338 | size fractionation | DBA Aurora | Developing grain tissue | durum | GSM5222604 | Control group |
| SRR14128337 | size fractionation | DBA Aurora | Developing grain tissue | durum | GSM5222603 | Heat stress group |
| SRR14128336 | size fractionation | DBA Aurora | Developing grain tissue | durum | GSM5222602 | Control group |
| SRR14128335 | size fractionation | DBA Aurora | Flag leaf tissue | durum | GSM5222601 | Heat stress group |
| SRR14128334 | size fractionation | DBA Aurora | Flag leaf tissue | durum | GSM5222600 | Control group |
| SRR14128333 | size fractionation | DBA Aurora | Flag leaf tissue | durum | GSM5222599 | Heat stress group |
| SRR14128332 | size fractionation | DBA Aurora | Flag leaf tissue | durum | GSM5222598 | Control group |
| Run | Library Name | Library Selection | Tissue | sub_species | Cultivar | Sample Name | Geographic Location Name | Treatment |
|---|---|---|---|---|---|---|---|---|
| SRR7226493 | svevomicro9 | RT-PCR | Grain | durum | Svevo | Svevo_miRNA_9 | Italy | - |
| SRR7226494 | svevomicro7 | RT-PCR | Rachis + glumes Leaf Epicotile + shoot Shoot Rootlet | durum | Svevo | Svevo_miRNA_7 | Italy | F. graminearum and F. culmorum inoculated spikes 36/72h or Zymoseptoria tritici Italian isolate inoculated seedlings 4h /1/2/9 d or Control spikes dd H2O 36/72h or control leaves ddH2O 4h / 1/2/9 d |
| SRR7226495 | svevomicro8 | RT-PCR | Shoot Root Leaf | durum | Svevo | Svevo_miRNA_8 | Italy | 1/4 Hoagland solution (HS) + 5mM KNO3 4/24/72 h / 7 d or 1/4 HS + K(H2PO4) 2mM 4/24/72 h / 7 d or 1/4 HS + 2.5 mM Mg(SO4) 4/24/72 h / 7 d or 1/4 HS + 100 µM Fe-EDTA 4/24/72 h / 7 d or 1/4 HS + 10mM (NH4)NO3 4/24/72 h / 7 d |
| SRR7226496 | svevomicro6 | RT-PCR | Coleoptile Root Culm node Spike Flag Leaf Glume + lemna Ovary + anther | durum | Svevo | Svevo_miRNA_6 | Italy | - |
| SRR7226499 | svevomicro5 | RT-PCR | Leaf Root | durum | Svevo | Svevo_miRNA_5 | Italy | 60µM CdCl2/2 d or 10µM ABA/4h or 50µM GA3/4h |
| SRR7226500 | svevomicro3 | RT-PCR | Leaf Root | durum | Svevo | Svevo_miRNA_3 | Italy | 25°C /20°C 12h light/12h dark or Nutrient supply/control: control 1/4 strength Hoagland solution then replenished with dd H2O |
| SRR7226498 | svevomicro4 | RT-PCR | Leaf Root | durum | Svevo | Svevo_miRNA_4 | Italy | 1/4 Hoagland solution + K(H2PO4) 2mM 4/24/72 h / 7 d or 1/4 Hoagland solution + 10mM (NH4)NO3 4/24/72 h / 7 d or UV exposure: 3h first day/2h second day/2h third day + recovery 24h or 10mM H2O2 2/4h |
| SRR7226501 | svevomicro1 | RT-PCR | Leaf Root Coleoptyle Spike | durum | Svevo | Svevo_miRNA_1 | Italy | 3°C/1.5°C 12h light /12h dark /24 h or 36°C/ 4h or 37°C/24h ,37°C/24h + 45°C/1h, 37°C/24h + 45°C/1h + 24°C/3d |
| SRR7226497 | svevomicro2 | RT-PCR | Leaf Root | durum | Svevo | Svevo_miRNA_2 | Italy | Drought/wounding 90 min/3h or 20% PEG 6000 3h/7h or 150mM NaCl 6h/24h |